ABSTRACT
O presente estudo comparou os níveis plasmáticos de glicose, proteínas totais, uréia, triglicerídeos e colesterol, a contagem de eritrócitos, leucócitos e trombócitos entre alevinos e juvenis de Arapaima gigas (Osteoglociformes, Arapaimidae) de uma piscicultura semi-intensiva de Manaus, estado do Amazonas, Brasil. Os alevinos de A. gigas apresentaram níveis significativamente (p<0,05) menores de proteínas totais, triglicerídeos, uréia e Volume Corpuscular Médio (VCM) e maior concentração de glicose, colesterol e hemoglobina, contagem de eritrócitos, hematócrito e Concentração da Hemoglobina Corpuscular Média (CHCM) quando comparados aos juvenis. Porém, não houve diferença significativa (p>0,05) no número de trombócitos e leucócitos totais entre alevinos e juvenis. Os juvenis de A. gigas apresentaram menor quantidade de linfócitos e maior quantidade de monócitos, neutrófilos e eosinófilos, quando comparados aos alevinos. Em estudos futuros, espécimes de A. gigas com outras idades também deverão ser comparados para melhor entendimento dos efeitos da idade neste peixe amazônico, pois estas informações, além de possibilitarem uma melhor compreensão da fisiologia desta espécie, poderão auxiliar no estabelecimento de estratégias para seu manejo.
The present study compared the biochemical and hematological parameters of fingerlings and juveniles Arapaima gigas (Osteoglociformes, Arapaimidae) from a semi-intensive fish farming in Manaus, Amazon State, Brazil. Fingerlings A. gigas had lower levels of total protein, triglycerides, urea and mean cell volume (MCV), but higher (p<0.05) glucose, cholesterol and hemoglobin concentration, red blood cell counts, hematocrit and cell hemoglobin concentration (MCHC) when compared to juveniles. However, there was not significant difference (p>0.05) in the total thrombocytes and total white blood cell counts between fingerlings and juveniles. Juveniles A. gigas presented lower lymphocytes number and higher monocytes, neutrophils and eosinophils number when compared to the fingerlings. In further studies, A. gigas specimens with other ages should also be compared for a better understanding of the ontogenic development in this Amazon fish, because this information besides increase the knowledge of the physiology of this species, can aid in the establishment of strategies for its adequate handling.
Subject(s)
Animals , Erythrocytes , Fishes/blood , Leukocytes , Amazonian Ecosystem , Fisheries , Hematologic Tests/veterinaryABSTRACT
The stingless bees of the Partamona genus have been studied taxonomically, ecologically and behaviourally, but cytogenetic studies are still rare. The objective of this study was to obtain cytogenetic data to contribute to Partamona peckolti species characterization. Heterochromatin was localized in all chromosome pericentromeric regions but some blocks could be visualized on some large chromosomes arms. A large heterozygous DA-CMA(3)-positive band was observed on one large chromosome arm, but was completely absent when C banding was applied before fluorochrome staining, with only one small positive band being visualized. Sequential DA-CMA(3)-NOR staining of interphase nuclei provided coincident positive responses. This suggests that DA-CMA(3)-positive bands of P. peckolti correspond to nucleolar organizer regions, as previously confirmed for another Partamona species by FISH